Modifying DNA

July 1, 2023

By Steven Henderson

Abstract: The present invention is directed to advanced genetic engineering techniques for modifying organisms that involve the use of programmable nucleases such as CRISPR/Cas9. The techniques provide a highly efficient and precise method for modifying the genetic material of organisms, allowing for the creation of new varieties with desirable traits. The advanced genetic engineering techniques comprise designing guide RNA sequences specific to the target DNA sequence, introducing the programmable nuclease to the organism, and allowing the nuclease to cleave the DNA at the target site. This induces a double-stranded break that can be repaired through either the non-homologous end joining or homology-directed repair pathway, resulting in the desired genetic modification. The advanced genetic engineering techniques have broad applications in agriculture, medicine, and biotechnology, and have the potential to significantly improve productivity and quality of life.

Claim 1: A method for modifying the genetic material of an organism using programmable nucleases, comprising designing guide RNA sequences specific to the target DNA sequence, introducing the programmable nuclease to the organism, and allowing the nuclease to cleave the DNA at the target site.

Claim 2: The method of claim 1, wherein the programmable nuclease is CRISPR/Cas9.

Claim 3: The method of claim 1, wherein the guide RNA sequences are designed to direct the programmable nuclease to a specific target site on the DNA.

Claim 4: The method of claim 1, wherein the double-stranded break induced by the programmable nuclease can be repaired through either the non-homologous end joining or homology-directed repair pathway.

Claim 5: The method of claim 1, wherein the genetic modification induced by the method results in a desired trait in the organism.

Claim 6: The method of claim 1, wherein the organism is a plant.

Claim 7: The method of claim 1, wherein the organism is an animal.

Claim 8: The method of claim 1, wherein the organism is a microorganism.

Claim 9: The method of claim 1, wherein the method is used for the production of proteins or other biologically active molecules.

Claim 10: The method of claim 1, wherein the method is used for the treatment of genetic diseases in humans.